미생물 작용을 이용한 법랑질 우식 및 재광화 평가
Microbiological Model for Enamel Remineralization and Demineralization
홍석진, 심영자, 박기철,
소속 상세정보
홍석진 ( ) - 전남대학교 치과대학 예방치과학교실
심영자 ( ) - Indiana University School of Dentistry Oral Health Research Institute
박기철 ( ) - 단국대학교 치과대학 예방치과학교실
KMID : 0355419960200040431
Abstract
The majority of study models on remineralization and demineralization reported in the literature are based on pH-cycle chemical systems. The purpose of this study was to develop a plaque covered demin/remin model. 60 bovine enamel cores were prepared and fixed on a plexiglass rods and randomly coded. Subsurface lesions were created by exposing the cores to a solution containing 0.1M lactic acid, 0.2% Carbopol, pH 5.0, with 50% HAP saturation for 35 hours. After surface hardness numbers (VHN) were determined, the specimens were assigned to 5 test groups of 12 specimens each by randomized blocks with VHN stratification. The specimens were sterilized-with ethylene oxide gas and exposed to filter-sterilized mixed human saliva for 24 hours under continuous stirring to form salivary pellicles. The 5 test groups were saline, human mixed. saliva, placebo, SMFP(1,100 ppm) /DCPD and NaF(1,100 ppm) /Silica dentifrices. The specimens were then exposed to Trypticase Soy Broth supplemented with 10% sucrose (TSB-S) and inoculated with Streptococci sobrinus for 2 days. The specimens were daily transferred in a freshly inoculated TSB-S. The specimens were provided with 3 daily 2-minute dentifrice treatments at 4 hour intervals in the daytime between demin (TSB-S; 2 hours) and remin (mixed human saliva /artificial saliva, 1:1; 2 hours) cycles. After the
last dentifrice slurry treatment in the evening every day, the specimens were exposed to remin/demin solution for 6 and 10 hours, respectively. These cycles were repeated for two weeks. The specimens then were cleaned under a stream of tap water and sterilized by exposing to sodium hypochloride solution for 5 minutes followed by distilled water rinse. The VHNs were randomly determined in the proximity of the initial areas tested of the same specimens and decoded by treatment groups. The changes of Mean VHN ± SD were; 2.17±3.70, 3.98±2.64, 13.18±7.90, 31.68±14.12, 56.11±14.12 from saline, saliva, Placebo, SMFP(1,100 ppm F)/DCPD, and NaF(1,100 ppm F)/Silica, respectively. These results were then statistically analyzed using ANOVA for the significance of differences among treatment groups. The results indicated that (1) the delta VHNs of both groups treated with fluoride dentifrices were significantly higher (p<0.05) than placebo, saline or saliva groups; (2) The VHN of NaF/Silica dentifrice was significantly higher (p<0.05) than SMFP/DCPD dentifrice; and (3) the study model can be utilized for comparing different dentifrices.
키워드
초기우식법랑질;미생물의 작용;재광화
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